A nuclease specific to lepidopteran insects suppresses RNAi
نویسندگان
چکیده
منابع مشابه
Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects
RNA interference (RNAi) has become a widely used reverse genetic tool to study gene function in eukaryotic organisms and is being developed as a technology for insect pest management. The efficiency of RNAi varies among organisms. Insects from different orders also display differential efficiency of RNAi, ranging from highly efficient (coleopterans) to very low efficient (lepidopterans). We inv...
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Little information is available on genome size diversity among insects, even in otherwise well-studied groups such as the Lepidoptera. In fact, only six lepidopteran species have been studied to date. The present study therefore represents the first attempt to survey genome size variation in this group, giving estimates for more than 50 species and increasing the coverage of the order to 15 fam...
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In agricultural systems, insect pests can cause crop damage mainly through loss in yield or quality resulting in a loss in profits for farmers. Worldwide pests cost billions of dollars due to damage and use of pesticides. Chemical pesticides are still the major approach for controlling insect pests, but they are associated with significant hazards to the environment and human health. The altern...
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We have identified a human nuclease that specifically cleaves four-stranded DNA stabilized by G quartets (G4 DNA). This nuclease, GQN1 (G quartet nuclease 1), cuts within the single-stranded region 5' of the barrel formed by stacked G quartets. GQN1 does not cleave duplex or single-stranded DNA, Holliday junctions, or G4 RNA. Cleavage depends on DNA structure and not on flanking sequence. Activ...
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BACKGROUND Despite evidence suggesting a role in plant defence, the use of plant lectins in crop protection has been hindered by their low and species-specific insecticidal activity. Snowdrop lectin (Galanthus nivalis agglutinin; GNA) is transported to the haemolymph of insects after oral ingestion, and can be used as a basis for novel insecticides. Recombinant proteins containing GNA expressed...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2018
ISSN: 0021-9258
DOI: 10.1074/jbc.ra117.001553